7SBE

Structure of the K. lactis telomerase RNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	140.370, 85.000, 48.420
Unit cell angles	90.00, 106.11, 90.00

Refinement procedure

Resolution	19.710 - 2.650
R-factor	0.2356
Rwork	0.233
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r4g
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.720
High resolution limit [Å]	2.650	2.650
Number of reflections	15880	1177
<I/σ(I)>	5.4
Completeness [%]	99.3
Redundancy	5.4
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14% PEG3350 and 200 mM Na.citrate