7SBD

Murine Fab/IgE in complex with profilin from Hevea brasieliensis (Hev b 8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	57.962, 77.468, 144.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.420 - 3.040
R-factor	0.2302
Rwork	0.228
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1BAF & 5FDS
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.420	3.152
High resolution limit [Å]	3.040	3.040
Rmerge		0.271
Number of reflections	12929	1181
<I/σ(I)>	7.12
Completeness [%]	99.0
Redundancy	2
CC(1/2)	0.995	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	2% Tacsimate, pH 6.0, 0.1 M Bis-Tris, pH 6.5, 20% w/v PEG3350