7SB6
Crystal Structure of Ancestral Mammalian Cadherin-23 EC1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 118.446, 168.834, 125.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.529 - 2.588 |
Rwork | 0.204 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2whv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.554 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.630 |
High resolution limit [Å] | 2.588 | 2.588 |
Rmerge | 0.091 | 1.022 |
Number of reflections | 39416 | 1925 |
<I/σ(I)> | 18.1 | 1.75 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 3.5 M Ammonium chloride 0.1 M MES pH 6.5 |