7SAL
Crystal Structure of LaM6 Nanobody bound to mCherry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-02 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.083, 87.948, 74.634 |
Unit cell angles | 90.00, 109.49, 90.00 |
Refinement procedure
Resolution | 49.970 - 1.930 |
R-factor | 0.1621 |
Rwork | 0.161 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h5q |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.990 |
High resolution limit [Å] | 1.920 | 4.140 | 1.920 |
Rmerge | 0.090 | 0.055 | 0.949 |
Rmeas | 0.097 | 0.059 | 1.046 |
Rpim | 0.034 | 0.020 | 0.425 |
Total number of observations | 492694 | ||
Number of reflections | 66672 | 6596 | 6712 |
<I/σ(I)> | 10.1 | ||
Completeness [%] | 98.8 | 95.8 | 99.1 |
Redundancy | 7.4 | 7.6 | 5.7 |
CC(1/2) | 0.997 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 100mM MIB buffer with 25% (w/v) PEG1500 |