7SAK

Crystal Structure of LaM4 Nanobody bound to mCherry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	156.554, 41.920, 47.827
Unit cell angles	90.00, 94.37, 90.00

Refinement procedure

Resolution	42.150 - 1.150
R-factor	0.1186
Rwork	0.117
R-free	0.14390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h5q
Data reduction software	DENZO (720)
Data scaling software	SCALEPACK (720)
Phasing software	PHASER (1.17)
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.000	75.000	1.190
High resolution limit [Å]	1.150	2.480	1.150
Rmerge	0.082	0.061	0.417
Rmeas	0.086	0.064	0.456
Rpim	0.027	0.020	0.181
Number of reflections	207109	11258	8475
<I/σ(I)>	11.2
Completeness [%]	97.0	99.9	77.3
Redundancy	9.6	10.3	5.9
CC(1/2)		0.998	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	295	20 % PEG3350, 100 mM MMT (Malic Acid, MES and TRIS)