7SAJ
Crystal Structure of LaM2 Nanobody bound to mCherry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.437, 160.560, 88.786 |
Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
Resolution | 71.000 - 2.370 |
R-factor | 0.2081 |
Rwork | 0.208 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h5q |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 71.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 4.850 | 2.250 |
Rmerge | 0.063 | 0.025 | 0.864 |
Rmeas | 0.074 | 0.030 | 1.019 |
Rpim | 0.039 | 0.015 | 0.534 |
Number of reflections | 106342 | 10781 | 10673 |
<I/σ(I)> | 8.9 | ||
Completeness [%] | 99.1 | 99.3 | 99.5 |
Redundancy | 3.6 | 3.6 | 3.6 |
CC(1/2) | 0.999 | 0.525 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 2.5M malonate |