7SAI
Crystal Structure of Lag30 Nanobody bound to eGFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 173.172, 173.172, 92.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.940 - 2.230 |
R-factor | 0.1724 |
Rwork | 0.171 |
R-free | 0.19710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eul |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.146 | 0.088 | 1.791 |
Rmeas | 0.151 | 0.092 | 1.859 |
Rpim | 0.041 | 0.025 | 0.496 |
Number of reflections | 65199 | 3624 | 3353 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.6 | 13 | 13.9 |
CC(1/2) | 0.997 | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 800 mM KH2PO4/NaH2PO4, 100 mM HEPES |