7SAH

Crystal Structure of LaG16 Nanobody bound to eGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	75.353, 88.238, 132.276
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.120 - 1.600
R-factor	0.1571
Rwork	0.156
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eul
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.056	0.028	1.054
Rmeas	0.060	0.030	1.160
Rpim	0.023	0.011	0.476
Total number of observations	393519
Number of reflections	58133	6073	5750
<I/σ(I)>	11.9
Completeness [%]	100.0	100	99.7
Redundancy	6.8	6.8	5.7
CC(1/2)		0.999	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	295	100mM BICINE with 2% (w/v) 1,4-Dioxane and 10% (w/v) PEG20000