7SAF
Fragment of streptococcal M87 protein fused to GCN4 adaptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 97.223, 97.223, 40.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.480 - 2.450 |
R-factor | 0.2581 |
Rwork | 0.257 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kql |
RMSD bond length | 0.007 |
RMSD bond angle | 0.870 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.480 | 2.538 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.387 | 4.684 |
Rmeas | 0.404 | 4.911 |
Rpim | 0.117 | 1.459 |
Number of reflections | 7580 | 738 |
<I/σ(I)> | 31.4 | 0.78 |
Completeness [%] | 99.8 | |
Redundancy | 12.1 | |
CC(1/2) | 0.987 | 0.556 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.6 M monobasic ammonium phosphate, 0.1 M Tris-HCl, pH 8.0 |