7S94
Structure of the core postfusion porcine endogenous retrovirus fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.860, 34.219, 100.445 |
Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
Resolution | 47.700 - 2.000 |
R-factor | 0.1767 |
Rwork | 0.174 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jgs |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.3) |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.730 | 47.730 | 2.050 |
High resolution limit [Å] | 2.000 | 8.930 | 2.000 |
Rmerge | 0.162 | 0.110 | 0.605 |
Rmeas | 0.175 | 0.117 | 0.688 |
Rpim | 0.066 | 0.041 | 0.316 |
Total number of observations | 4114 | 8291 | |
Number of reflections | 41923 | 566 | 1970 |
<I/σ(I)> | 7.3 | 11.2 | 3.1 |
Completeness [%] | 93.4 | 98.9 | 60.4 |
Redundancy | 6.6 | 7.3 | 4.2 |
CC(1/2) | 0.984 | 0.991 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 24% (w/v) PEG 1500 and 20% (v/v) glycerol |