7S8T

M. xanthus ferritin-like protein EncC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	148.703, 47.797, 135.330
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	37.770 - 2.490
R-factor	0.3341
Rwork	0.325
R-free	0.33950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n5e
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.770	2.580
High resolution limit [Å]	2.490	2.490
Rmeas	0.160	0.540
Number of reflections	32526	3099
<I/σ(I)>	12.5	6.7
Completeness [%]	96.4	92.9
Redundancy	6.6
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	291.15	20% PEG MME, 10% PEG 20000, 100 mM Tris-Bicine pH 8.5, 30 mM calcium chloride, 5 mM iron chloride