7S8S
Crystal Structure of HLA A*1101 in complex with RVLVNGTFLK, an 10-mer epitope from Influenza B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-17 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.875, 82.262, 57.027 |
| Unit cell angles | 90.00, 113.04, 90.00 |
Refinement procedure
| Resolution | 25.390 - 1.870 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.125 | 1.183 |
| Rmeas | 0.135 | |
| Rpim | 0.052 | 0.481 |
| Total number of observations | 265223 | |
| Number of reflections | 38748 | 2267 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.997 | 0.682 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 26% PEG 3350, 0.1M BisTris pH 6.3, 0.2M Lithium Sulfate |
