7S7Q

Heterodimeric complex of Pf12 and Pf41 of Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953644
Spacegroup name	P 43 21 2
Unit cell lengths	73.712, 73.712, 332.241
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.160 - 2.850
R-factor	0.2327
Rwork	0.230
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ymo 4ys4
RMSD bond length	0.005
RMSD bond angle	0.807
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.355	49.355	3.020
High resolution limit [Å]	2.850	8.430	2.850
Rmerge	0.076	0.039	1.044
Rmeas	0.081	0.041	1.117
Total number of observations	322715
Number of reflections	40863	1578	6458
<I/σ(I)>	15.59	46.07	1.54
Completeness [%]	99.7	99.1	98.2
Redundancy	7.897	7.421	7.952
CC(1/2)	0.999	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	10 mM cadmium chloride, 14% (w/v) PEG 4000, 25% glycerol, 50 mM HEPES pH 7.5