7S6T

Complex structure of Methane monooxygenase hydroxylase and regulatory subunit H33A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	102.520, 105.600, 299.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.510 - 1.820
R-factor	0.1475
Rwork	0.146
R-free	0.17220
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7m8q
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.510	1.850
High resolution limit [Å]	1.820	1.820
Rmerge	0.094	1.079
Rmeas	0.105	1.200
Rpim	0.046	0.519
Total number of observations	1472673
Number of reflections	289411	13878
<I/σ(I)>	9.3
Completeness [%]	99.5	97.2
Redundancy	5.1	5.2
CC(1/2)	0.996	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	21% PEG3350 and 0.2 M Na2HPO4 pH 6.6