7S6N

N-acetylglucosamine-1-phosphotransferase (GNPT) alpha and beta subunits (GNPTAB) catalytic domain, from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.18
Spacegroup name	P 1 21 1
Unit cell lengths	97.182, 86.605, 106.985
Unit cell angles	90.00, 112.17, 90.00

Refinement procedure

Resolution	48.420 - 2.700
R-factor	0.1858
Rwork	0.183
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold-2
RMSD bond length	0.005
RMSD bond angle	0.642
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmeas	0.115
Number of reflections	63354	4471
<I/σ(I)>	16.5
Completeness [%]	90.5
Redundancy	6.6
CC(1/2)		0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% PEG3350, 0.1M HEPES pH 7.5