7S65
Compressed conformation of nighttime state KaiC
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Number of Particle | 2016826 |
Software | Appion,RELION,Coot,Leginon,UCSF Chimera |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |