7S5C
M. xanthus ferritin-like protein EncB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.830, 68.360, 83.390 |
| Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
| Resolution | 43.340 - 1.860 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n5e |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.761 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmeas | 0.090 | 0.870 |
| Number of reflections | 56065 | 4286 |
| <I/σ(I)> | 12.8 | 1.4 |
| Completeness [%] | 95.4 | 71.5 |
| Redundancy | 6.2 | 3.2 |
| CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291.15 | 20% PEG MME, 10% PEG 20000, 100 mM Tris-Bicine pH 8.5, 30 mM calcium chloride |
