7S58
Crystal Structure of the tick evasin EVA-P974 complexed to human chemokine CCL7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.025, 64.415, 181.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.770 - 1.820 |
R-factor | 0.2246 |
Rwork | 0.224 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s4n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.004 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.880 | 1.850 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 61992 | 3397 |
<I/σ(I)> | 18.5 | |
Completeness [%] | 99.4 | |
Redundancy | 13.4 | |
CC(1/2) | 0.999 | 0.678 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BIS-TRIS 6.5 pH (Buffer) 25 %w/v PEG 3350 (Precipitant) |