7S4Y
Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-06-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.27819 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.600, 81.600, 33.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.350 - 1.710 |
| R-factor | 0.24199 |
| Rwork | 0.240 |
| R-free | 0.25774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ey9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.077 |
| Data reduction software | CrystFEL (v0.10.0) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 9140 | 928 |
| <I/σ(I)> | 7.9 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 1292 | 881 |
| CC(1/2) | 0.979 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | insulin was dissolved in 5 mM zinc chloride, 25 mM HCl at a concentration of 5-10 mg/ml. Cuboid-shaped microcrystals were obtained in 35.2 mM sodium citrate pH 7, and 5% (v/v) acetone as precipitant |
