7S42
Crystal structure of an N-acetyltransferase from Helicobacter pullorum in the presence of Coenzyme A and dTDP-3-acetamido-3,6-dideoxy-D-galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-09 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.438, 103.438, 103.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.880 - 1.350 |
R-factor | 0.1325 |
Rwork | 0.132 |
R-free | 0.14850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s3u |
RMSD bond length | 0.010 |
RMSD bond angle | 1.625 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.450 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 40420 | 7761 |
<I/σ(I)> | 23.6 | 6.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.4 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 11-18% PEG-3350, 200 mM KCl, 5 mM dTDP-3-amino-3,6-dideoxy-D-galactose, 5 mM acetyl coenzyme A, 100 mM HEPPS |