7S3L
Structure of Bacillus subtilis CcrZ (previously called YtmP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9784 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.118, 98.118, 69.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.690 - 2.600 |
R-factor | 0.2445 |
Rwork | 0.242 |
R-free | 0.26290 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.039 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.120 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.146 | 1.598 |
Number of reflections | 10890 | 1299 |
<I/σ(I)> | 14.8 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25.5 | 27.2 |
CC(1/2) | 0.998 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.84 | 298 | 30% PEG 6000, 1 M LiCl, 0.1 M NaOAc (pH 5.84) |