7S3B

Crystal structure of intact U2AF65 RRM-region bound to AdML-G5 oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97945
Spacegroup name	P 21 21 21
Unit cell lengths	43.668, 57.839, 76.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.850 - 1.890
R-factor	0.1751
Rwork	0.173
R-free	0.19980
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	Aimless (0.5.17)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.470	1.930
High resolution limit [Å]	1.890	1.890
Rmerge	0.105	0.377
Rpim	0.062	0.232
Number of reflections	29672	908
<I/σ(I)>	10.1	2.9
Completeness [%]	99.2	89.3
Redundancy	6.9	5.8
CC(1/2)	0.995	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.24 M Na Malonate, 26% Peg 3350, n-dodecyl-N,N-dimethylamine-N-oxide (=LDAO)