7S3B
Crystal structure of intact U2AF65 RRM-region bound to AdML-G5 oligonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.668, 57.839, 76.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.850 - 1.890 |
R-factor | 0.1751 |
Rwork | 0.173 |
R-free | 0.19980 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.470 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.105 | 0.377 |
Rpim | 0.062 | 0.232 |
Number of reflections | 29672 | 908 |
<I/σ(I)> | 10.1 | 2.9 |
Completeness [%] | 99.2 | 89.3 |
Redundancy | 6.9 | 5.8 |
CC(1/2) | 0.995 | 0.922 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.24 M Na Malonate, 26% Peg 3350, n-dodecyl-N,N-dimethylamine-N-oxide (=LDAO) |