7S2V

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-12
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	77.433, 77.433, 37.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.410 - 1.600
R-factor	0.192
Rwork	0.190
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.011
RMSD bond angle	1.644
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	13.410	13.410	1.630
High resolution limit [Å]	1.600	8.620	1.600
Rmerge	0.051	0.024	0.511
Total number of observations	76667	488	2376
Number of reflections	15331	93	761
<I/σ(I)>	16.3	44.8	2.1
Completeness [%]	99.3	74.1	97.7
Redundancy	5	5.2	3.1
CC(1/2)	0.999	0.999	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection