7S2E
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS 3.0 MICROFOCUS |
| Temperature [K] | 125 |
| Detector technology | CMOS |
| Collection date | 2020-12-12 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.335, 78.335, 36.919 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.160 - 1.600 |
| R-factor | 0.1827 |
| Rwork | 0.181 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qwy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | SAINT (V8.40A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 22.160 | 22.160 | 1.657 |
| High resolution limit [Å] | 1.600 | 3.443 | 1.600 |
| Number of reflections | 29921 | 3110 | 29921 |
| <I/σ(I)> | 8.56 | ||
| Completeness [%] | 99.9 | ||
| Redundancy | 7.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
