7S23
Crystal structure of alpha-COP-WD40 domain, Y139A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.315, 171.553, 71.281 |
Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
Resolution | 65.020 - 1.490 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.18400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j87 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.696 |
Data reduction software | HKL-3000 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 171.550 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.086 | 0.710 |
Number of reflections | 140251 | 6840 |
<I/σ(I)> | 5 | 1.1 |
Completeness [%] | 97.9 | |
Redundancy | 3.6 | |
CC(1/2) | 0.996 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 18% PEG3350 and 0.2M Potassium-sodium tartarate |