7S1U

Structure of human POT1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-26
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	162.880, 162.880, 63.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 - 2.550
R-factor	0.2022
Rwork	0.185
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5un7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.620
High resolution limit [Å]	2.550	2.550
Number of reflections	31601	2261
<I/σ(I)>	19.69
Completeness [%]	99.7
Redundancy	11
CC(1/2)	0.999	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	800 mM ammonium phosphate pH 8.1, 24% w./v. PEG 4,000 and 7% 2-Methyl-2,4-pentanediol