7S1T

Structure of the human POT1-TPP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-19
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 1
Unit cell lengths	70.880, 70.980, 103.790
Unit cell angles	76.76, 84.61, 70.55

Refinement procedure

Resolution	36.440 - 2.900
R-factor	0.2532
Rwork	0.250
R-free	0.30690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5un7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.440	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	36930	2747
<I/σ(I)>	9.32
Completeness [%]	90.0
Redundancy	5.9
CC(1/2)	0.996	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1M HEPES pH 7.5 and 3.0M NaCl