7S1P

PRMT5/MEP50 crystal structure with sinefungin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	96.755, 137.730, 178.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 2.210
R-factor	0.2111
Rwork	0.209
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s0u
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.160	2.400
High resolution limit [Å]	2.200	2.200
Number of reflections	41740	444
<I/σ(I)>	9.5	0.9
Completeness [%]	69.5
Redundancy	2.1
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		281	100 mM Sodium Citrate pH 5.4, 15% PEG 3350, 4% Tacsimate pH 5