7S1O
Structure of human POT1C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-19 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.980, 61.800, 83.740 |
Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
Resolution | 28.980 - 2.550 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5un7 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.620 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 20551 | 1266 |
<I/σ(I)> | 13.92 | |
Completeness [%] | 98.3 | |
Redundancy | 7.3 | |
CC(1/2) | 0.970 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 200 mM DL-Malic acid pH 7.0, 30% v./v. PEG600 and 6% methanol |