7S16

Crystal structure of alpha-COP-WD40 domain R57A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	35.868, 56.712, 70.548
Unit cell angles	90.00, 99.37, 90.00

Refinement procedure

Resolution	43.970 - 1.240
R-factor	0.136
Rwork	0.135
R-free	0.15900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j87
RMSD bond length	0.005
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.610	1.260
High resolution limit [Å]	1.240	1.240
Rmerge	5.400
Number of reflections	74665	2292
<I/σ(I)>	11.5
Completeness [%]	94.2
Redundancy	4.3
CC(1/2)	0.998	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	22% PEG 3350 and 0.2M tri-sodium citrate