7S08
Crystal structure of Epstein-Barr virus gH/gL targeting antibody 770F7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.194, 40.222, 135.151 |
Unit cell angles | 90.00, 110.54, 90.00 |
Refinement procedure
Resolution | 44.620 - 2.420 |
R-factor | 0.2148 |
Rwork | 0.212 |
R-free | 0.26040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qhk |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 22877 | 1928 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 95.4 | |
Redundancy | 4.5 | |
CC(1/2) | 0.850 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES PH7.5, 0.1M CaCl2, 25% PEG3350, 4% isopropanol |