7S01
X-ray structure of the phage AR9 non-virion RNA polymerase holoenzyme in complex with a forked oligonucleotide containing the P077 promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-13 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.91840 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 176.925, 110.455, 222.379 |
Unit cell angles | 90.00, 98.52, 90.00 |
Refinement procedure
Resolution | 49.350 - 3.400 |
R-factor | 0.242 |
Rwork | 0.239 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EMD-24763 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.469 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.580 |
High resolution limit [Å] | 3.380 | 3.380 |
Number of reflections | 59745 | |
<I/σ(I)> | 7.94 | 1.2 |
Completeness [%] | 94.0 | 83.9 |
Redundancy | 2.88 | 2.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.5 ul aliquot of the AR9 nvRNAP promoter complex (10 mg/mL) was mixed with the same volume of a solution containing 150 mM MIB pH 5, 150 mM LiCl, 13 % PEG 1500 and incubated as a hanging drop over the same solution. |