7S00
X-ray structure of the phage AR9 non-virion RNA polymerase core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-24 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.858, 166.270, 307.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.420 - 3.300 |
R-factor | 0.2208 |
Rwork | 0.219 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7s01 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.575 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.740 | 3.500 |
High resolution limit [Å] | 3.300 | 3.500 | 3.300 |
Rmeas | 0.139 | 0.757 | 1.420 |
Number of reflections | 87373 | 13096 | 13885 |
<I/σ(I)> | 10.07 | 2.39 | 1.17 |
Completeness [%] | 98.6 | 99.3 | 98.7 |
Redundancy | 4.49 | 4.15 | |
CC(1/2) | 0.998 | 0.774 | 0.421 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | an 1.5 ul aliquot of AR9 nvRNAP core (4.5 mg/mL) was mixed with an equal volume of a solution containing 100 mM Tricine pH 8.8, 270 mM KNO3, 15 % PEG 6000, 5 mM MgCl2, and incubated as a hanging drop over the same solution |