7RZZ
Crystal structure of FBF-2 in complex with LST-1 site A peptide and compact FBE RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 93.169, 93.169, 111.278 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.700 - 2.390 |
R-factor | 0.1751 |
Rwork | 0.171 |
R-free | 0.21510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k5q |
RMSD bond length | 0.002 |
RMSD bond angle | 0.446 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.700 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rpim | 0.031 | 0.186 |
Number of reflections | 26102 | 2118 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% (v/v) PEG 400, 0.1 M CHES, pH 9.5 |