7RZM

Crystal Structure of dnaN DNA polymerase III beta subunit from Stenotrophomonas maltophilia K279a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 62
Unit cell lengths	160.960, 160.960, 36.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.470 - 2.150
R-factor	0.1719
Rwork	0.168
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tsz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.470	46.470	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.078	0.021	0.586
Rmeas	0.087	0.024	0.655
Total number of observations	148980
Number of reflections	29740	372	2182
<I/σ(I)>	16.99	49.34	2.94
Completeness [%]	99.9	97.4	100
Redundancy	5.009	4.344	5.021
CC(1/2)	0.999	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	287	MORPHEUS F5 (321505f5): 100 mM MOPS/HEPES-Na pH 7.5, 20 mM D-glucose, 20 mM D-mannose, 20 mM D-galactose, 20 mM L-fucose, 20mM D-xylose, 20 mM N-acetyl-D-glucosamine, 10% (w/v) PEG 20000, 20% (v/v) PEG MME 550, 16.74 mg/mL StmaA.17987.a.B1.PS38645, direct cryo