7RZ2
Hen egg-white lysozyme with ionic liquid ethanolammonium formate 4 mol%
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.760, 76.760, 38.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.290 - 1.070 |
| Rwork | 0.152 |
| R-free | 0.16970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jmu |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.283 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.290 | 1.090 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Number of reflections | 50880 | 2520 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | ethanolammonium formate 4mol% (ca. 20wt%) in water |
