7RYW
Crystal structure of Ferritin grown by the microbatch method in the presence of Agarose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-02 |
Detector | ADSC HF-4M |
Wavelength(s) | 0.979 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 181.507, 181.507, 181.507 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.700 - 2.090 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.19580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w0o |
RMSD bond length | 0.006 |
RMSD bond angle | 0.773 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.700 | 2.150 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.112 | |
Number of reflections | 15632 | 1090 |
<I/σ(I)> | 0.935 | |
Completeness [%] | 99.8 | |
Redundancy | 20 | |
CC(1/2) | 1.000 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 290 | CdSO4, NH4SO4, Tris pH 7.4 |