7RY2
mSandy2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.680, 147.210, 240.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.500 - 2.050 |
R-factor | 0.1913 |
Rwork | 0.190 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h5q |
RMSD bond length | 0.003 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.310 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rpim | 0.052 | 0.890 |
Number of reflections | 133939 | 6432 |
<I/σ(I)> | 11.9 | 1 |
Completeness [%] | 99.9 | 97.3 |
Redundancy | 13 | 10.9 |
CC(1/2) | 0.999 | 0.396 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris buffer (pH 8.5), 100 mM NaCl, 20% PEG-3350, 34 mg/mL protein |