7RY1
human Hsp90_MC domain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 159.850, 311.964, 88.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.421 - 3.523 |
| R-factor | 0.2204 |
| Rwork | 0.216 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q6m |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.806 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
| Rmerge | 0.148 | 0.055 | 1.366 |
| Rmeas | 0.161 | 0.060 | 1.487 |
| Rpim | 0.063 | 0.023 | 0.581 |
| Total number of observations | 176259 | ||
| Number of reflections | 27438 | 2902 | 2708 |
| <I/σ(I)> | 3.9 | ||
| Completeness [%] | 99.9 | 99.8 | 100 |
| Redundancy | 6.4 | 6.1 | 6.4 |
| CC(1/2) | 0.998 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Succinic acid PH 6.5, 12% PEG3350, 20mM CaCl2 |
