7RX6
Structure of METTL3-METTL14(R298C) mutant methyltransferase complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97938 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 99.743, 99.743, 118.378 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.870 - 1.800 |
R-factor | 0.1941 |
Rwork | 0.193 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k7m |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.796 | 1.796 |
Rmerge | 0.100 | 1.080 |
Number of reflections | 56123 | 5499 |
<I/σ(I)> | 23.6 | |
Completeness [%] | 100.0 | |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris (pH 8.0) and 20% PEG3350 |