7RWM

Structure of Cap5 from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-24
Detector	RAYONIX MX300-HS
Wavelength(s)	0.97872
Spacegroup name	P 43
Unit cell lengths	180.772, 180.772, 93.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.190 - 3.400
R-factor	0.251
Rwork	0.249
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rwk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.061
Number of reflections	41618	4116
<I/σ(I)>	9.2
Completeness [%]	100.0
Redundancy	7.6
CC(1/2)	0.980	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	12-15% PEG6000, 100 mM sodium citrate, pH 5.5, 100 mM NaCl