7RWK

Structure of Cap5 from Asticcacaulis sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	80
Detector technology	CCD
Collection date	2020-12-14
Detector	RAYONIX MX300HE
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	69.311, 96.922, 131.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.720 - 2.390
R-factor	0.1957
Rwork	0.193
R-free	0.23530
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.390	2.400
Rmerge	0.069	0.368
Number of reflections	35476	3305
<I/σ(I)>	10.3
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)	0.990	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	15-17% PEG6000, 100 mM Tris-HCl, pH 8.5, 100 mM NaCl