7RW4
Crystal structure of junctophilin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.076, 55.203, 142.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.480 - 1.310 |
R-factor | 0.1381 |
Rwork | 0.137 |
R-free | 0.16800 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.340 |
High resolution limit [Å] | 1.310 | 3.230 | 1.310 |
Rmerge | 0.073 | 0.048 | 1.097 |
Rmeas | 0.079 | 0.052 | 1.228 |
Rpim | 0.031 | 0.021 | 0.542 |
Total number of observations | 528933 | ||
Number of reflections | 84406 | 6008 | 5455 |
<I/σ(I)> | 8.5 | ||
Completeness [%] | 99.7 | 99.8 | 97.6 |
Redundancy | 6.3 | 6.1 | 5 |
CC(1/2) | 0.996 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium Acetate |