7RW4

Crystal structure of junctophilin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	44.076, 55.203, 142.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.480 - 1.310
R-factor	0.1381
Rwork	0.137
R-free	0.16800
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.340
High resolution limit [Å]	1.310	3.230	1.310
Rmerge	0.073	0.048	1.097
Rmeas	0.079	0.052	1.228
Rpim	0.031	0.021	0.542
Total number of observations	528933
Number of reflections	84406	6008	5455
<I/σ(I)>	8.5
Completeness [%]	99.7	99.8	97.6
Redundancy	6.3	6.1	5
CC(1/2)		0.996	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium Acetate