7RVL

Segment from the Y169F mutant of the human prion protein 168-176 EFSNQNNFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-04-25
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.900, 10.380, 30.260
Unit cell angles	90.91, 90.82, 102.25

Refinement procedure

Resolution	10.140 - 1.000
R-factor	0.1915
Rwork	0.190
R-free	0.21060
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	10.140	10.140	1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.178	0.097	0.363
Rmeas	0.197	0.107	0.400
Total number of observations	14661
Number of reflections	2737	29	163
<I/σ(I)>	5.99	10.18	3.66
Completeness [%]	87.1	80.6	69.7
Redundancy	5.357	5.517	5.006
CC(1/2)	0.985	0.986	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1 M sodium acetate, pH 4.5, 1 M sodium chloride, 0.1 M lithium sulfate