7RVK
Segment from Y169G mutant of the human prion protein 169-175 GSNQNNF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ELECTRON MICROSCOPE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-09-22 |
Detector | TVIPS TEMCAM-F416 |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 |
Unit cell lengths | 4.860, 14.110, 18.410 |
Unit cell angles | 90.00, 93.71, 101.21 |
Refinement procedure
Resolution | 13.840 - 1.000 |
R-factor | 0.2024 |
Rwork | 0.198 |
R-free | 0.23820 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.010 |
RMSD bond angle | 0.940 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 13.840 | 13.840 | 1.030 |
High resolution limit [Å] | 1.000 | 4.470 | 1.000 |
Rmerge | 0.238 | 0.159 | 0.430 |
Rmeas | 0.257 | 0.171 | 0.485 |
Total number of observations | 12907 | ||
Number of reflections | 2076 | 18 | 123 |
<I/σ(I)> | 5.05 | 7.72 | 2.25 |
Completeness [%] | 80.3 | 69.2 | 64.7 |
Redundancy | 6.217 | 6.722 | 4.26 |
CC(1/2) | 0.980 | 0.976 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% w/v PEG8000, 0.1 M MES, pH 6, 0.2 M sodium acetate |