7RVG

Segment from rabbit/pig prion protein 168-176 QYSNQNSFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-03-22
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1 21 1
Unit cell lengths	23.570, 4.860, 27.700
Unit cell angles	90.00, 111.21, 90.00

Refinement procedure

Resolution	7.180 - 1.000
R-factor	0.2184
Rwork	0.218
R-free	0.21960
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.180	7.180	1.030
High resolution limit [Å]	1.000	4.470	1.000
Rmerge	0.204	0.107	0.698
Rmeas	0.220	0.113	0.767
Total number of observations	19765
Number of reflections	2680	25	180
<I/σ(I)>	5.72	11.31	2.45
Completeness [%]	74.9	43.1	71.7
Redundancy	7.375	5.88	6.628
CC(1/2)	0.994	0.999	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	0.05 M CHES, pH 9, 0.2 M lithium sulfate, 1.3 M sodium/potassium tartrate