7RVF

Segment from the Y169F mutant of the bank vole prion protein 168-176 QFNNQNNFV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Source details	OTHER
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-03-22
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 1
Unit cell lengths	4.880, 10.560, 29.980
Unit cell angles	93.89, 92.38, 103.29

Refinement procedure

Resolution	10.240 - 1.000
R-factor	0.2202
Rwork	0.217
R-free	0.25060
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	1.662
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	10.240	10.240	1.030
High resolution limit [Å]	1.000	4.490	1.000
Rmerge	0.229	0.142	0.636
Rmeas	0.248	0.151	0.700
Total number of observations	18329
Number of reflections	2938	29	123
<I/σ(I)>	5.33	10.28	2.28
Completeness [%]	94.8	87.9	54.9
Redundancy	6.239	6.483	5.154
CC(1/2)	0.980	0.988	0.711

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.2 M MES, pH 6, 10% MPD