7RVE
Segment from the S170N mutant of the human prion protein 168-176 EYNNQNNFV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ELECTRON MICROSCOPE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-04-25 |
Detector | TVIPS TEMCAM-F416 |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 |
Unit cell lengths | 4.930, 10.140, 31.560 |
Unit cell angles | 94.13, 90.59, 102.74 |
Refinement procedure
Resolution | 9.860 - 0.850 |
R-factor | 0.2177 |
Rwork | 0.213 |
R-free | 0.26630 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 9.860 | 9.860 | 0.870 |
High resolution limit [Å] | 0.850 | 3.800 | 0.850 |
Rmerge | 0.185 | 0.139 | 0.505 |
Rmeas | 0.211 | 0.156 | 0.631 |
Total number of observations | 16649 | ||
Number of reflections | 4694 | 54 | 161 |
<I/σ(I)> | 3.81 | 8.43 | 1.22 |
Completeness [%] | 89.8 | 87.1 | 43.2 |
Redundancy | 3.547 | 4.241 | 2.435 |
CC(1/2) | 0.985 | 0.972 | 0.617 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1 M sodium acetate, pH 4.5, 1 M sodium chloride, 0.1 M lithium sulfate |