7RUS

Crystal structure of the octameric form of Rv3208A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	120
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	54.411, 79.456, 119.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.890 - 2.870
R-factor	0.1991
Rwork	0.198
R-free	0.22510
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.647
Data reduction software	HKL-2000 (1.18.2_3874)
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.890	2.973
High resolution limit [Å]	2.870	2.870
Rmerge	0.164
Number of reflections	11983	1143
<I/σ(I)>	34.42
Completeness [%]	96.9
Redundancy	8.3
CC(1/2)	0.980	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES (pH 7.5) and 10 % (w/v) PEG 8000, 8 % (v/v) Ethylene Glycol.