7RUO

Crystal structure of human UTP15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-04
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	58.327, 59.085, 61.868
Unit cell angles	90.11, 66.82, 62.87

Refinement procedure

Resolution	42.690 - 1.800
R-factor	0.2331
Rwork	0.231
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.490
Data scaling software	Aimless (0.7.4)
Phasing software	BALBES
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.470	42.660	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.108	0.067	0.464
Rmeas	0.145	0.089	0.625
Rpim	0.096	0.059	0.415
Total number of observations		1015	7198
Number of reflections	57233	481	3260
<I/σ(I)>	4.7	7.7	2
Completeness [%]	93.7	97.2	90
Redundancy	2.2	2.1	2.2
CC(1/2)	0.982	0.953	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	2.0M (NH4)2SO4, 0.2M Sodium acetate, 0.1M Hepes pH7.5, 5% MPD